Multilocus Sequence Typing* (MLST) is a nucleotide sequence based approach for the unambiguous characterisation of isolates of microbial species via the internet

MLST provides a portable, unambiguous, and discriminatory typing system that is very widely used for many bacterial and fungal pathogens, both for molecular epidemiology and population and evolutionary biology



MLST was developed in 1998 by adapting the proven concepts of multilocus enzyme electrophoresis (MLEE) so that alleles at multiple loci (typically seven) are defined directly, by nucleotide sequencing, rather than indirectly from the electrophoretic moblity of their gene products.

*Maiden, M.C.J., Bygraves, J.A., Feil, E., Morelli, G., Russell, J.E., Urwin, R., Zhang, Q., Zhou, J., Zurth, K., Caugant, D.A., Feavers, I.M., Achtman, M., and Spratt, B.G.  1998. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms.  Proc. Natl. Acad. Sci. USA, 95: 3140-3145.



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