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Instructions

Introduction

MLST-Maps
    eBURST
    Trees

Database Query
    Results
    Maps

    eBURST
    Trees
    Download Data

Locus Query
    Sinlgle

    Multiple
    Batch

ST & Profile Query
    Sinlgle
    Batch
    ST Query

eBURST
    User data
    Geographic

Downloading Data

Organism Specific

Submitting Data
    alleles
    Sequence Types
    Isolate data
    Next-gen data

 

Policy Document

eBURST - User Data NEEDS WORK

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The User Data eBURST option allows you to run eBURST on your own MLST data. You can also compare your own MLST data with those in the MLST database.

 

The first screen of the 'Enter your data' option provides a form for entering your own MLST data.

 

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Data must be entered in comma- or TAB-delimited format - example data in either format are available by clicking the 'Click here for example data' buttons.

The data for each isolate you enter should start with the ST number, followed by the alleles at the seven MLST loci.

After pasting your data you can choose one of three options:

1) Analyse your allelic profiles separately: This will launch eBURST containing only your query data
2) Add your allelic profiles to a list of the allelic profiles of all isolates in the database
3) Add your allelic profiles to a list of distinct STs (i.e. one of each ST in the MLST database)

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NOTE: As eBURST can show the abundance of isolates of each ST in your dataset (both in tabular form and from the size of the circles representing each ST in the eBURST diagram), using Option 1 above you should enter the ST and allelic profile of all isolates in your dataset and not just one of each ST.

Once you have pasted your data, and chosen your option, hit 'Click and Wait' - an eBURST icon will appear below the form, indicating your option and providing a link to launch eBURST:

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In the next section we describe the use of geographic eBURST to compare isolates in the MLST database from different countries.

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